Translational_Unit
Part:BBa_K3994011:Design
Designed by: Nixue Song Group: iGEM21_NOFLS_YZ (2021-10-12)
PyeaR_HrpR+PttB344_HrpS_PJ23105_ttrR+PhrpL_amilGFP_IL10_PJ23104_ttrS
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1248
Illegal XbaI site found at 1398
Illegal XbaI site found at 3288
Illegal XbaI site found at 3438
Illegal PstI site found at 1236
Illegal PstI site found at 3276 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2062
Illegal NheI site found at 2085
Illegal NheI site found at 5496
Illegal NheI site found at 5519
Illegal PstI site found at 1236
Illegal PstI site found at 3276 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3116
Illegal BamHI site found at 1254
Illegal BamHI site found at 3294 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1248
Illegal XbaI site found at 1398
Illegal XbaI site found at 3288
Illegal XbaI site found at 3438
Illegal PstI site found at 1236
Illegal PstI site found at 3276 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1248
Illegal XbaI site found at 1398
Illegal XbaI site found at 3288
Illegal XbaI site found at 3438
Illegal PstI site found at 1236
Illegal PstI site found at 3276
Illegal NgoMIV site found at 669
Illegal AgeI site found at 142
Illegal AgeI site found at 256
Illegal AgeI site found at 262
Illegal AgeI site found at 901
Illegal AgeI site found at 1776
Illegal AgeI site found at 2329
Illegal AgeI site found at 2335 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
No
Source
No